Selected References – Microfluidics
R. Hernández Vera, E. Schwan, N. Fatsis-Kavalopoulos, J. Kreuger
This study presents a cost-effective solution for time-lapse imaging, including a climate-controlled incubation chamber, based on 3D-printed elements, a smartphone and off-the-shelf electronics.Read more
E. Berthier and D. J. Beebe
Gradient generation platforms have been one of the few applications of microfluidics that have begun to be translated to biological laboratories and may become a new “gold standard”. Though gradient generation platforms are now established, their full potential has not yet been realized.Read more
Sackmann EK et al.
This study identifies neutrophil chemotaxis velocity as a potential biomarker for asthma, and the authors demonstrate a microfluidic technology that was used in a clinical setting to perform these measurements.Read more
Sackmann EK, Fulton AL and Beebe DJ
Microfluidics, a technology characterized by the engineered manipulation of fluids at the submillimetre scale, has shown considerable promise for improving diagnostics and biology research. The authors suggest directions that biologists, engineers and clinicians can take to help this technology live up to its full potential.Read more
Junkin M and Tay S.
Understanding immunity in the face of complexity and noisy dynamics requires time-dependent analysis of single-cells in a proper context. Microfluidic systems create precisely defined microenvironments by controlling fluidic and surface chemistries, feature sizes, geometries and signal input timing, and thus enable quantitative multi-parameter analysis of single cells. Such qualities allow observable dynamic environments approaching in vivo levels of biological complexity.Read more
Mehling M and Tay S.
Microfluidic techniques allow precise control of fluids and particles at the nanoliter scale and facilitate simultaneous manipulation and analysis of cultured cells, starting from a single cell to larger populations and to intact tissues. The use of integrated microfluidic devices has considerably advanced the fields of quantitative and systems biology.Read more
Nguyen NT, Shaegh SAM, Kashaninejad N and Phan DT
Lab-on-a-chip technology is an emerging field evolving from the recent advances of micro- and nanotechnologies. At the cellular level, a concentration gradient generator integrated with a cell culture platform is the main drug delivery scheme of interest.Read more
Wu J. Wu X and Lin F.
Microfluidic devices can better control cellular microenvironments compared to conventional cell migration assays. Over the past few years, microfluidics-based chemotaxis studies showed a rapid growth. New strategies were developed to explore cell migration in manipulated chemical gradients.Read more
Zhang C, Jang S, Amadi O, Shimizu K, Lee R and Mitchell RN
Existing chemotaxis assays do not generate stable chemotactic gradients and thus—over time—functionally measure only nonspecific random motion (chemokinesis). In comparison, microfluidic technology has the capacity to generate a tightly controlled microenvironment that can be stably maintained for extended periods of time and is, therefore, amenable to adaptation for assaying chemotaxis.Read more
Wong KHK, Chan JM, Kamm RD and Tien J.
In vitro studies of vascular physiology have traditionally relied on cultures of endothelial cells, smooth muscle cells, and pericytes grown on centimeter-scale plates, filters, and flow chambers. The introduction of microfluidic tools has revolutionized the study of vascular physiology by allowing researchers to create physiologically relevant culture models, at the same time greatly reducing the consumption of expensive reagents.Read more
Roussos ET, Condeelis JS and Patsialou A.
Chemotaxis of tumour cells and stromal cells in the surrounding microenvironment is an essential component of tumour dissemination during progression and metastasis. The central importance of chemotaxis in cancer progression is highlighted by discussion of the use of chemotaxis as a prognostic marker, a treatment end point and a target of therapeutic intervention.Read more
Beta C and Bodenschatz E.
Over the past decade, microfluidic techniques have been established as a versatile platform to perform live cell experiments under well-controlled conditions. To investigate the directional responses of cells, stable concentration profiles of chemotactic factors can be generated in microfluidic gradient mixers that provide a high degree of spatial control.Read more
Ahmed T, Shimizu TS and Stocker R.
The latest generation of microfluidic gradient generators, in particular, holds appeal for both biophysicists seeking to unravel the fundamental mechanisms of bacterial chemotaxis, and ecologists wishing to model chemotaxis in realistic environments.Read more
Kim S, Kim HJ and Jeon NL.
This review discusses the emergence of microfluidics-based gradient generators and their applications in enhancing our understanding of fundamental biological processes such as chemotaxis and morphogenesis.Read more
Wu MH, Huang SB, Lee GB.
Due to the significant differences in several physical phenomena between microscale and macroscale devices, microfluidic technology provides unique functionality, which is not previously possible by using traditional techniques.