Morphogenesis and advanced chemotaxis

CellDirector® 3D


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The ability of cells to respond to gradients is essential to most aspects of animal development and to disease progression. CellDirector 3D is the first commercially available assay for generation of controlled concentration gradients in true three-dimensional (3D) cell cultures. Study morphology or advanced chemotaxis of complex samples like cell spheroids in response to gradients of your signalling molecules. Excellent cell culture conditions allow for time-lapse imaging of cell behavior during experiments exceeding days.


Popular research applications of CellDirector® 3D

  • Organ morphogenesis
  • Initiation and suppression of angiogenesis
  • Determination of MIC and dynamic sub-MIC studies of microorganisms
  • Stem cell migration and differentiation
    Application protocols and guides

CellDirector 3D_webCells and matrix are injected into CellDirector 3D and a steady-state
linear concentration gradient is formed by diffusion.


Benefits of CellDirector® 3D

  • Stable and well-established linear gradients
  • Allows culture and response studies of small organs and spheroids
  • Excellent cell culture conditions
  • Reduces time to publication
    Product documentation and manuals   

Technical information

Cell culture volume 8-9 µl
Time to steady-state gradient 12 h for e.g. VEGFA
2 h for e.g. ampicillin
Bottom thickness #1.5 (170 µm)
Bottom material D263™ M borosilicate glass
Transmission wave lengths 300-1200 nm
 Sterilized by Ethylene oxide
Catalogue# (10 assays/box) REF 10-001-10



C. Malmberg, M.Sc.Eng. (Antibiotics Research Unit, Uppsala University Hospital)
“The CellDirector 3D assay enables us to explore the pharmacodynamics of antibiotics with unprecedented resolution, due to the precise and highly repeatable linear concentration gradient!”