Selected References – Microfluidics

Current Opinion in Cell Biology 2017, 48:72–78

Mechanisms for fast cell migration in complex environments

Vargas P, Barbier L, Sáez PJ, Matthieu Piel

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PLoS ONE, 2016

A Modular and Affordable Time-Lapse Imaging and Incubation System Based on 3D-Printed Parts, a Smartphone, and Off-The-Shelf Electronics

R. Hernández Vera, E. Schwan, N. Fatsis-Kavalopoulos, J. Kreuger

This study presents a cost-effective solution for time-lapse imaging, including a climate-controlled incubation chamber, based on 3D-printed elements, a smartphone and off-the-shelf electronics.

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Lab on a Chip, 2014 - Frontier

Gradient generation platforms: new directions for an established microfluidic technology

E. Berthier and D. J. Beebe

Gradient generation platforms have been one of the few applications of microfluidics that have begun to be translated to biological laboratories and may become a new “gold standard”. Though gradient generation platforms are now established, their full potential has not yet been realized.

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PNAS, 2014

Characterizing asthma from a drop of blood using neutrophil chemotaxis

Sackmann EK et al.

This study identifies neutrophil chemotaxis velocity as a potential biomarker for asthma, and the authors demonstrate a microfluidic technology that was used in a clinical setting to perform these measurements.

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Nature, 2014 - Review

The present and future role of microfluidics in biomedical research

Sackmann EK, Fulton AL and Beebe DJ

Microfluidics, a technology characterized by the engineered manipulation of fluids at the submillimetre scale, has shown considerable promise for improving diagnostics and biology research. The authors suggest directions that biologists, engineers and clinicians can take to help this technology live up to its full potential.

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Lab on Chip, 2014 - Critical Review

Microfluidic single-cell analysis for systems immunology

Junkin M and Tay S.

Understanding immunity in the face of complexity and noisy dynamics requires time-dependent analysis of single-cells in a proper context. Microfluidic systems create precisely defined microenvironments by controlling fluidic and surface chemistries, feature sizes, geometries and signal input timing, and thus enable quantitative multi-parameter analysis of single cells. Such qualities allow observable dynamic environments approaching in vivo levels of biological complexity.

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Current Opinions in Biotechnology, 2014 - Review

Microfluidic cell culture

Mehling M and Tay S.

Microfluidic techniques allow precise control of fluids and particles at the nanoliter scale and facilitate simultaneous manipulation and analysis of cultured cells, starting from a single cell to larger populations and to intact tissues. The use of integrated microfluidic devices has considerably advanced the fields of quantitative and systems biology.

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Advanced Drug Delivery Reviews, 2013

Design, fabrication and characterization of drug delivery systems based on lab-on-chip technology

Nguyen NT, Shaegh SAM, Kashaninejad N and Phan DT

Lab-on-a-chip technology is an emerging field evolving from the recent advances of micro- and nanotechnologies. At the cellular level, a concentration gradient generator integrated with a cell culture platform is the main drug delivery scheme of interest.

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Lab on a Chip, 2013 - Critical Review

Recent developments in microfluidics-based chemotaxis studies

Wu J. Wu X and Lin F.

Microfluidic devices can better control cellular microenvironments compared to conventional cell migration assays. Over the past few years, microfluidics-based chemotaxis studies showed a rapid growth. New strategies were developed to explore cell migration in manipulated chemical gradients.

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BioMed Research International, 2013 - Clinical Study

A sensitive chemotaxis assay using a novel microfluidic device

Zhang C, Jang S, Amadi O, Shimizu K, Lee R and Mitchell RN

Existing chemotaxis assays do not generate stable chemotactic gradients and thus—over time—functionally measure only nonspecific random motion (chemokinesis). In comparison, microfluidic technology has the capacity to generate a tightly controlled microenvironment that can be stably maintained for extended periods of time and is, therefore, amenable to adaptation for assaying chemotaxis.

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Annual Reviews of Biomedical Engineering, 2012

Microfluidic models of vascular functions

Wong KHK, Chan JM, Kamm RD and Tien J.

In vitro studies of vascular physiology have traditionally relied on cultures of endothelial cells, smooth muscle cells, and pericytes grown on centimeter-scale plates, filters, and flow chambers. The introduction of microfluidic tools has revolutionized the study of vascular physiology by allowing researchers to create physiologically relevant culture models, at the same time greatly reducing the consumption of expensive reagents.

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Nature Reviews, 2011

Chemotaxis in cancer

Roussos ET, Condeelis JS and Patsialou A.

Chemotaxis of tumour cells and stromal cells in the surrounding microenvironment is an essential component of tumour dissemination during progression and metastasis. The central importance of chemotaxis in cancer progression is highlighted by discussion of the use of chemotaxis as a prognostic marker, a treatment end point and a target of therapeutic intervention.

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European Journal of Cell Biology, 2011 - Review

Microfluidic tools for quantitative studies of eukaryotic chemotaxis

Beta C and Bodenschatz E.

Over the past decade, microfluidic techniques have been established as a versatile platform to perform live cell experiments under well-controlled conditions. To investigate the directional responses of cells, stable concentration profiles of chemotactic factors can be generated in microfluidic gradient mixers that provide a high degree of spatial control.

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Integrative Biology, 2010 - Critical Review

Microfluidics for bacterial chemotaxis

Ahmed T, Shimizu TS and Stocker R.

The latest generation of microfluidic gradient generators, in particular, holds appeal for both biophysicists seeking to unravel the fundamental mechanisms of bacterial chemotaxis, and ecologists wishing to model chemotaxis in realistic environments.

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Integrated Biology, 2010 - Critical Review

Biological applications of microfluidic gradient devices

Kim S, Kim HJ and Jeon NL.

This review discusses the emergence of microfluidics-based gradient generators and their applications in enhancing our understanding of fundamental biological processes such as chemotaxis and morphogenesis.

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Lab on a Chip, 2010 - Tutorial Review

Microfluidic cell culture systems for drug research

Wu MH, Huang SB, Lee GB.

Due to the significant differences in several physical phenomena between microscale and macroscale devices, microfluidic technology provides unique functionality, which is not previously possible by using traditional techniques.


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